Biochemistry (9th Edition)

Chapter 5

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Techniques employed for solubilizing a protein are... more

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When it is necessary to rupture cells while ... more

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Salting out can be defined as a protein ... more

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The composition and amino acid arrangement in ... more

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Homogenization of liver cells using Potter–... more

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Differential centrifugation is a technique in ... more

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The proteins present within the cell cytoplasm can... more

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The first step required for isolating the ... more

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For isolating the protein required from the ... more

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The cells from which the desired proteins of the ... more

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Proteins are eluted in decreasing order in the gel... more

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Compounds can be eluted in the following two ways:... more

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Elution of a compound may occur in the following ... more

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Increasing the salt concentration does not affect ... more

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Dextran or agarose Polyacrylamide ; The technique ... more

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Cation exchanger:Anion exchanger:   ; Ion-exchange... more

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The size range of the gel-filtration column can be... more

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Both the proteins(enzymes) would pass through ... more

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Sephadex  G-75 has an exclusion limit of 80,000 ... more

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Reverse-phase HPLCIon-exchange chromatographyThe ... more

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Basis of differenceHigh-performance liquid ... more

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For purification of the given protein, an anion ... more

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The stability of pH while using the quaternary ... more

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Firstly, the organelles should be separated ... more

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Glutamic acidYes ; A cation exchange resin is ... more

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Phenylalanine will exhibit fast mobility while ... more

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Glutamic acidPhenylalanine ; Polar substances ... more

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The protein solution that is mixed with or ... more

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ChargeSizeShape ; Electrophoresis is the process ... more

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Agarose and polyacrylamide. ; Electrophoresis is ... more

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Polyacrylamide ; Electrophoresis is the process of... more

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Nucleic acid ; Electrophoresis is the process of ... more

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The molecules that are negatively charged and are ... more

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Sodium dodecyl sulfate polyacrylamide-gel ... more

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SDS is added to protein before adding it to the ... more

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The polymer is in the form of a solid block in gel... more

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32000 Da ; In SDS-PAGE (Sodium dodecyl-sulfate ... more

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Edman degradation has made the process of ... more

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It helps to know the number of polypeptide ... more

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; PITC or phenylisothiocyanate is the Edman ... more

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Due to degradation of the analyzed signals. ; ... more

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The first two amino acids would be released from ... more

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; The enzyme trypsin cuts the peptide chain at ... more

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; Proteins are polymers composed of a few to ... more

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The C-terminal in the first trial is asparagine, ... more

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The C-terminal of the fragments should end with ... more

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Sequencing can be done in a better way if the ... more

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Cyanogen bromide ; The enzymes that are normally ... more

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Chymotrypsin would be the most useful enzyme for ... more

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Chymotrypsin would be the most effective if ... more

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Electrospray ionization mass spectrometry (ESI-MS... more

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Highly accurate and sensitive.Attomole quantities ... more

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Antibody-protein interaction. ; ELISA is a ... more

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The antibodies that bind to protein directly are ... more

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The complex of antibody and protein can be ... more

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Separation of proteins injected into the gel using... more

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The name of western blot is derived from the ... more

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Advantage: Compared to the microarray technique, ... more

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The nitrocellulose membrane requires less amount ... more

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To reduce the cost of protein identification and ... more

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Proteomics is the analysis of the complete set of ... more

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The affinity tag on the bait protein helps it to ... more

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The major assumption is that the binding of the ... more

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The protein can be separated using a cation ... more

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